单个植入前胚胎构建cDNA文库

以PCR为基础的单个植入前胚胎cDNA文库构建是一种快速、可重复和高效的建库方法。单个植入前胚胎cDNA文库作为一种重要的新兴基因资源库 ,为新基因的克隆和鉴定奠定了坚实的基础 ,不仅解决了胚胎研究材料受限的问题 ,而且在时间上更加精确 ,更符合胚胎发育的规律。是研究早期胚胎发育基因表达的一种有效手段。随着这一技术的不断改进和与其它技术的有机结合 ,必然为人们进一步揭示胚胎发育的分子机制开创一个崭新的局面。

Construction cDNA Library from Single preimplantation Embryo

Construction of PCR\|based cDNA libraries form single preimplantation embryos is a rapid,reproducible and efficient method.This library provides a new and important resource for the cloning and identification of novel genes or gene families.It not only overcome the difficulty of scarcity sample but also reflect temporally more precise molecular developmental mechanism.As development and improvement constantly of this technique,it will disclose molecular mechanism of embryo development.