Total RNA isolation from<Emphasis Type="Italic">Picea mariana</Emphasis> dry seed |
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| Authors: | Helen H Tai Céline Pelletier Tannis Beardmore |
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| Institution: | (1) Canadian Forest Service, Natural Resources Canada, PO Box 4000, E3B 5P7 Fredericton, New Brunswick, Canada |
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| Abstract: | Isolating RNA from dry conifer seeds can be difficult because of a number of interfering compounds present in seeds. We describe
a protocol for total RNA isolation from black spruce dry seeds, which is an adaptation of a method used for mouse myeloma
tissues. The extraction relies on selective precipitation of RNA by using lithium chloride. |
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| Keywords: | conifer lithium chloride Picea RNA isolation seed spruce |
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