Sildenafil citrate induces migration of mouse aortic endothelial cells and proteinase secretion |
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Authors: | Young Il Kim In Suk Oh Seung Moon Park Hwan Gyu Kim |
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Institution: | 1. Department of Physical Education, Yonsei University, 120-749, Seoul, Korea 2. Division of Biological Sciences, Research Center of Bioactive Materials, Chonbuk National University, 561-756, Jeonju, Korea
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Abstract: | Vascular endothelial cells release proteinases that degrade the extracellular matrix (ECM), thus enabling cell migration during
angiogenesis and vasculogenesis. Sildenafil citrate stimulates the nitric oxide-cyclic guanosine monophosphate pathway through
inhibition of phosphodiesterase type V (PDE5). In this report, we examined the mechanisms underlying sildenafil citrate-induced
cell migration using cultured mouse aortic endothelial cells (MAECs). Sildenafil citrate induced migration and proteinase
secretion by murine endothelial cells. Sildenafil citrate induced the secretion of matrix metalloproteinase-2 (MMP-2) and
MMP-9, which is inhibited by NF-κB inhibitors. Sildenafil citrate also induced the secretion of plasmin, which is inhibited
by Pl 3′-kinase inhibitors. It is suggested that sildenafil citrate-induced migrating activity in endothelial cells may be
accomplished by increased secretion of proteinases. |
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