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Ion-exchange high-performance liquid chromatography of oligodeoxyribonucleotides using formamide |
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| Authors: | C R Newton A R Greene G R Heathcliffe T C Atkinson D Holland A F Markham M D Edge |
| Institution: | 1. ICI Pharmaceuticals Division, Alderley Park, Macclesfield, Cheshire SK10 4TG, United Kingdom;2. Corporate Bioscience Group, Alderley Park, Macclesfield, Cheshire SK10 4TG, United Kingdom |
| Abstract: | The superiority of buffer systems containing formamide for the ion-exchange high-performance liquid chromatographic separation of oligodeoxyribonucleotide mixtures generated in solid-phase syntheses is illustrated. The resolutions achieved are compared to those achieved with the same mixtures in other eluting solvents. The use of formamide systems is recommended for oligodeoxyribonucleotide purification in general and is particularly valuable where the oligonucleotide of interest is highly self-complementary and/or rich in deoxyguanosine residues. |
| Keywords: | synthetic oligonucleotide ion-exchange high-performance liquid chromatography formamide solid-phase sequence effects |
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