Uranyl salts as photochemical agents for cleavage of DNA and probing of protein-DNA contacts |
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| Authors: | P E Nielsen C Jeppesen O Buchardt |
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| Affiliation: | Department of Biochemistry B, Panum Institute, University of Copenhagen, Denmark. |
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| Abstract: | Single-strand DNA nicks are induced by uranyl nitrate or uranyl acetate in combination with long-wavelength (lambda approximately 420 nm) ultraviolet irradiation. The nicks occur randomly with respect to the DNA sequence. Using the lambda-repressor/ORI operator DNA system it is shown that uranyl salts can be used to photofootprint protein contacts with the DNA backbone. |
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