Simultaneous determination of felbamate and four metabolites in rat cerebrospinal fluid by high-performance liquid chromatography |
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Authors: | L. A. Romanyshyn J. K. Wichmann N. Kucharczyk R. D. Sofia |
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Affiliation: | Department of Biochemistry, Wallace Laboratories, Division of Carter-Wallace, Half Acre Road, Cranbury, NJ 08512 USA |
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Abstract: | An isocratic liquid chromatographic method for direct sample injection has been developed for the quantitation of felbamate and four metabolites in rat cerebrospinal fluid. The method uses 0.050- or 0.025-ml aliquots of cerebrospinal fluid diluted with equal volumes of internal standard. Chromatography is performed on a 150 mm × 4.6 mm I.D. Spherisorb ODS2, 3-μm HPLC column eluted with a phosphate buffer—acetonitrile—methanol (820:120:60, v/v/v) mobile phase and ultraviolet absorbance detection at 210 nm. The linear quantitation ranges are: felbamate and the 2-hydroxy metabolite 0.195–200 μg/ml, the propionic acid metabolite 0.195–50.0 μg/ml, the p-hydroxy metabolite 0.781 to 50.0 μg/ml, and the monocarbamate metabolite 0.098–50.0 μg/ml. |
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