Stimulation of glycolysis by imidazole

Imidazole stimulated glycolysis by rat diaphragm homogenates; this effect increased as the concentration of imidazole was raised. The simultaneous changes in concentration of some of the glycolytic intermediates suggested an action of imidazole at a step between triose phosphate and pyruvate. Addition of glyceraldehyde phosphate dehydrogenase increased glycolysis and abolished the stimulating effect of imidazole, thus leading to the conclusion that in the homogenate the activity of this enzyme was rate-limiting for glycolysis and was increased by imidazole.