Co-lyophilization of bovine serum albumin (BSA) with poly(ethylene glycol) improves efficiency of BSA encapsulation and stability in polyester microspheres by a solid-in-oil-in-oil technique |
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Authors: | Wasfi Al-Azzam Emil A Pastrana Kai Griebenow |
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Institution: | (1) Departments of Biology, University of Puerto Rico, Río Piedras Campus, P.O. Box 23346, San Juan, PR 00931-3346, USA;(2) Chemistry, University of Puerto Rico, Río Piedras Campus, P.O. Box 23346, San Juan, PR 00931-3346, USA |
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Abstract: | The effect of the co-lyophilization of bovine serum albumin (BSA) with poly(ethylene glycol) (PEG) on the BSA encapsulation efficiency and formation of soluble BSA aggregates upon solid-in-oil-in-oil (s/o/o) encapsulation in poly(lactic-co-glycolic) acid (PLGA) microspheres was investigated. Suspension of the lyophilized BSA-PEG formulations in methylene chloride produced small protein powder particles of less than 1 m diam. and this afforded high encapsulation efficiencies of typically 90% ameliorating one of the problems in s/o/o encapsulation. Formation of soluble BSA aggregates upon s/o/o encapsulation followed by 24 h in vitro release was between 5% and 22%, much lower than values of 59% reported for BSA without stabilizing excipients. Therefore, PEG also afforded BSA stabilization during s/o/o encapsulation. Sustained release occurred over ca. 2 months and was complete. |
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Keywords: | drug delivery protein aggregation protein stability sustained release |
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