Affiliation: | * Department of Genetics and Molecular Biology, University of Rome “La Sapienza”, Via Lancisi 29, 00161, Rome, Italy † Institute of Parasitology, University of Rome “La Sapienza”, Piazzale Aldo Moro 5,00185, Rome, Italy ‡ Department of Agriculture and Fisheries for Scotland, Marine Laboratory, P.O. Box 101, Victoria Road, Aberdeen AB9 8DB, U.K. |
Abstract: | Nascetti G., Paggi L., Orecchia P., Smith J.W., Mattiucci S. and Bullini L.1986. Electrophoretic studies on the Anisakis simplex complex (Ascaridida: Anisakidae) from the Mediterranean and North-East Atlantic. International Journal for Parasitology 16: 633–640. The genetic variation of the sibling species Anisakis simplex A and A. simplex B was investigated by electrophoretic analysis of 22 gene-enzyme systems. The two species are reproductively isolated and no gene flow takes place between them. Three loci, Sod, Adk-2 and Lap-1, show distinct alleles in A. simplex A and A. simplex B, allowing their reliable identification both at the larval and adult stages. A fourth locus, Got, appears to be diagnostic at the 95% level. The value of Nei's genetic distance found between A. simplex A and A. simplex B is 0.28. Parameters of genetic variability (He, P, A) are given for both species. The geographic distribution of A. simplex A and A. simplex B appears to be mainly Mediterranean for the former, and mainly North Atlantic for the latter. Several paratenic hosts (fish and squid) and one cetacean definitive host are identified for each of the two species. The names A. pegreffii and A. simplex are tentatively proposed for A. simplex A and A. simplex B respectively. |