中华蜜蜂工蜂视叶胚后发育过程中的细胞凋亡

本研究通过形态解剖和原位末端标记法(TUNEL),对中华蜜蜂Apis cerana cerana视叶胚后发育过程中的细胞凋亡进行了研究,结果表明:视叶内的细胞程序性死亡开始出现在1龄幼虫末期,随后凋亡细胞数量逐渐增加;在视叶的胚后发育过程中,细胞凋亡经历了3个高峰期,即2龄幼虫、5龄幼虫和蛹发育的第2天;在视叶3个部分的发育中,视髓层中细胞凋亡的数量远远多于视小叶和视神经节层,而视神经节层最少,说明了细胞凋亡的数量和位置与各部分结构发育的时间以及神经投射有关。广泛的细胞凋亡是蜜蜂视叶发育过程中的一个显著特征。

Programmed cell death of developing optic lobes in the honey bee, Apis cerana cerana

Programmed cell death （ PCD） during the postembryonic development of the optic lobes of Apis cerana cerana was investigated by comparative anatomical research and TdT-mediated dUTP nick end labeling （TUNEL）. The results show that PCD begins within the brain after the end of the final larval instar after which the number of apoptotic nuclei increases. Apoptosis of cells in the optic lobes peaked in three developmental stages; 2 instar larvae,5 instar larvae and day 2 of pupal development. There was more apoptosis in the medulla than in the lobula and lamina. Nerve projection pulses trigger cell proliferation and apoptosis during optic lobe development.