Control of angiotensinogen production by H4 rat hepatoma cells in serum-free culture |
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Authors: | Masatoshi Togami Daphne Blazka Jun Hayashi |
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Institution: | (1) Japan Synthetic Rubber Co., Ltd., Kanawa, Japan;(2) W. Alton Jones Cell Science Center, Inc., 10 Old Barn Road, 12946 Lake Placid, New York |
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Abstract: | Summary A serum-free, hormone and attachment factor supplemented culture for rat H4 hepatoma cells was established. In the defined
medium (Dulbecco's Modified Eagle's +Ham's F12+insulin, transferrin, fibronectin liver cell growth factor, and sodium selenite),
H4 cells grew equally well as in 10% fetal bovine serum supplemented medium. H4 cells in either defined or serum-containing
culture conditions produce transferrin but not albumin or alpha-fetoprotein. In this paper we have studied the effect of various
hormones and pressor peptides on the production of angiotensinogen by H4 cells cultured in defined conditions. Only glucocorticoid
hormone had a significant effect on the production of angiotensinogen, whereas other hormones previously reported to exert
their effect on angiotensinogen production had little or no effect.
This work was supported by grant P01 CA37589 from the National Institutes of Health, Bethesda, MD. |
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Keywords: | H4 rat hepatoma angiotensinogen serum-free culture glucocorticoid |
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