Moesin is a biomarker for the assessment of genotoxic carcinogens in mouse lymphoma |
| |
Authors: | Yoen Jung Lee In-Kwon Choi Yhun Yhong Sheen Sue Nie Park Ho Jeong Kwon |
| |
Institution: | Department of Biotechnology, Yonsei University, Seoul, 120-749, Korea. |
| |
Abstract: | 1,2-Dibromoethane and glycidol are well known genotoxic carcinogens, which have been widely used in industry. To identify
a specific biomarker for these carcinogens in cells, the cellular proteome of L5178Y mouse lymphoma cells treated with these
compounds was analyzed by 2-dimensional gel electrophoresis (2-DE) and MALDI-TOF mass spectrometry (MS). Of 50 protein spots
showing a greater than 1.5-fold increase or decrease in intensity compared to control cells on a 2-D gel, we focused on the
candidate biomarker moesin. Western analysis using monoclonal rabbit anti-moesin confirmed the identity of the protein and
its increased level of expression upon exposure to the carcinogenic compounds. Moesin expression also increased in cells treated
with six additional genotoxic carcinogens, verifying that moesin could serve as a biomarker to monitor phenotypic change upon
exposure to genotoxic carcinogens in L5178Y mouse lymphoma cells. |
| |
Keywords: | biomarker genotoxic carcinogen moesin toxicoproteomics |
本文献已被 PubMed SpringerLink 等数据库收录! |
|