The purification and characterization of a novel hypersensitive-like response-inducing elicitor from Verticillium dahliae that induces resistance responses in tobacco |
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Authors: | Bingnan Wang Xiufen Yang Hongmei Zeng Hua Liu Tingting Zhou Beibei Tan Jingjing Yuan Lihua Guo Dewen Qiu |
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Institution: | (1) Key Laboratory for Biological Control of Ministry of Agriculture, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, No. 12 Zhong-guancun South Street, Beijing, 100081, People’s Republic of China; |
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Abstract: | PevD1, a novel protein elicitor from the pathogenic cotton verticillium wilt fungus, Verticillium dahliae, induced a hypersensitive response in tobacco plants. In this paper, the elicitor was purified and analyzed using de novo
sequencing. The protein-encoding pevD1 gene consists of a 468-bp open reading frame that produces a polypeptide of 155 amino acids, with a theoretical molecular
weight of 16.23 kDa. The sequence of elicitor protein PevD1 was matched to the genomic sequence (GenBank accession no. ABJE
01000445.1) of a putative protein from V. dahliae strain vdls.17, but a function had not yet been reported. The pevD1 gene was expressed in Escherichia coli, and the recombinant protein was characterized for its ability to confer systemic acquired resistance to tobacco mosaic virus
(TMV). Recombinant PevD1-treated plants exhibited enhanced systemic resistance compared to control, including a significant
reduction in the number and size of TMV lesions on tobacco leaves. The elicitor protein-induced hydrogen peroxide production,
extracellular-medium alkalization, callose deposition, phenolics metabolism, and lignin synthesis in tobacco. Our results
demonstrate that elicitor-PevD1 triggers defense responses in intact tobacco plants. |
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