Characterization of excitation beam on second-harmonic generation in fibrillous type I collagen |
| |
Authors: | Ying Chang Xiaoyuan Deng |
| |
Institution: | (1) MOE Key Laboratory of Laser Life Science, South China Normal University, Guangzhou, 510631, Guangdong, China |
| |
Abstract: | Following our established theoretical model to deal with the second-harmonic generation (SHG) excited by a linearly polarized
focused beam in type I collagen, in this paper, we further quantitatively characterize the differences between SHG emissions
in type I collagen excited by collimated and focused beams. The effects of the linear polarization angle (α) and the fibril polarity characterized by the hyperpolarizability ratio ρ on SHG emission has been compared under collimated and focused beam excitation, respectively. In particular, SHG emission
components along the i axis ( I2w,i )\left( {I_{2\omega {,}i} } \right) (i = x,y,z), the induced SHG emission deviation angle γ
ij
, and the detected SHG signals (I
2ω,ij
) in the ij plane by rotating the applied polarizer angle φ
ij
have been investigated (i = x, x, y; j = y, z, z). Results show that under our simulation model, SHG emission in the xy plane, such as I
2ω,x
,I
2ω,y
,γ
xy
and I
2ω,xy
varying as polarization angle (α) under collimated and focused light, presents no significant difference. The reverse of the fibril polarity has induced great
impact on I
2ω,x
,γ
xy
and I
2ω,xy
in both collimated and focused light. I
2ω,x
and γ
xy
show similarity, but I
2ω,xy
at α = 30° demonstrates a slight difference in focused light to that in collimated light. Under focused light, the reverse of
fibril polarity causes obvious changes of the collected SHG intensity I
2ω,xz
and I
2ω,yz
at a special polarization angle α = 60° and γ
xz
, γ
yz
along α. |
| |
Keywords: | Collimated beam Focused beam Polarization (α) SHG intensity |
本文献已被 PubMed SpringerLink 等数据库收录! |
|