Glutamic acid production in an airlift reactor with net draft tube

Cultivation of Brevibacterium divaricatum for glutamic acid production in an airlift reactor with net draft tube was developed. Cell concentration gave an index for adding penicillin G. On-line estimation of total sugar concentration yielded an identified model which was used for determination of the substrate addition. Fermentation for glutamic acid production requires high oxygen concentration in the broth. The proposed reactor has the capability to provide sufficient oxygen for the fermentation. Since the reactor is suitable for fed-batch culture, the cultivation of B. divaricatum for glutamic acid production in the proposed reactor is successfully carried out.List of Symbols a system parameter - b system parameter - C _c,in mole fraction carbon dioxide in the gas inlet - C _c,out mole fraction carbon dioxide in the gas outlet - C _L mole/dm³ oxygen concentration in liquid phase - C _L ^* mole/dm³ saturated oxygen concentration in liquid phase - C _0,in mole fraction of oxygen in the gas inlet - C _0,out mole fraction of oxygen in the gas outlet - CPR mole/h/dm³ carbon dioxide production rate based on total broth - E(t) error signal - F _in mole/h inlet gas flow rate - k ₁ constant defined by Eq. (4) - k ₂ constant defined by Eq. (5) - k _L a 1/h volumetric mass transfer coefficient of gas-liquid phase - OUR mole/h/dm³ oxygen uptake rate based on total broth - P atm pressure in the reactor - t h time - TS _c g total sugar consumption - TS _s g/dm³ set point of total sugar concentration - TS _* g/dm³ reference value of total sugar concentration - TS(t) g/dm³ total sugar concentration in the broth at timet - u(t) cm³/min feed rate at timet - V dm³ total broth volume - VVM (dm³/min)/dm³ flow rate per unit liquid volume - a negative constant defined by Eq. (7)