| Affiliation: | (1) Department of Urology, University of Oklahoma Health Sciences Center, Oklahoma City, OK, USA;(2) Department of Pathology, University of Oklahoma Health Sciences Center, Oklahoma City, OK, USA;(3) Department of Veterans Affairs Medical Center, Oklahoma City, OK, USA |
| Abstract: | Background Androgens and androgen receptors (AR) regulate normal prostate development and growth. They also are involved in pathological development of prostatic diseases, including benign prostatic hyperplasia (BPH) and prostate cancer (PCa). Antiandrogen therapy for PCa, in conjunction with chemical or surgical castration, offers initial positive responses and leads to massive prostate cell death. However, cancer cells later appear as androgen-independent PCa. To investigate the role of AR in prostate cell proliferation and survival, we introduced a vector-based small interfering RNA (siRNA). This siRNA targeted 5'-untranslated region of AR mRNA for extended suppression of AR expression in androgen-sensitive human prostate LNCaP cells. |
