Cloning of the koi herpesvirus (KHV) gene encoding thymidine kinase and its use for a highly sensitive PCR based diagnosis期刊界 All Journals 搜尽天下杂志传播学术成果专业期刊搜索期刊信息化学术搜索

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Cloning of the koi herpesvirus (KHV) gene encoding thymidine kinase and its use for a highly sensitive PCR based diagnosis

Authors:	Email author" target="_blank">Herve?Bercovier Email author Yolanta?Fishman Ronen?Nahary Sharon?Sinai Amir?Zlotkin Marina?Eyngor Oren?Gilad Avi?Eldar Ronald?P?Hedrick

Institution:	(1) Institute of Microbiology, Department of Clinical Microbiology, The Hebrew University-Hadassah Medical School, Ein Karen, Jerusalem, Israel;(2) Department of Poultry and Fish Diseases, The Kimron Veterinary Institute, Beit Dagan, Israel;(3) Department of Medicine and Epidemiology, School of Veterinary Medicine, University of California, 95616 Davis, California, USA;(4) Department of Infectious diseases, Sheba medical center, 52621 Tel Hashomer, Israel

Abstract:	Background Outbreaks with mass mortality among common carp Cyprinus carpio carpio and koi Cyprinus carpio koi have occurred worldwide since 1998. The herpes-like virus isolated from diseased fish is different from Herpesvirus cyprini and channel catfish virus and was accordingly designated koi herpesvirus (KHV). Diagnosis of KHV infection based on viral isolation and current PCR assays has a limited sensitivity and therefore new tools for the diagnosis of KHV infections are necessary.

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