Isolation and characterization of lipopeptide antibiotics produced by Bacillus subtilis |
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Authors: | H Chen L Wang CX Su GH Gong P Wang ZL Yu |
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Institution: | Key Laboratory of Ion Beam Bioengineering, Chinese Academy of Sciences, Hefei, Anhui, China |
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Abstract: | Aims: Antibiotics from Bacillus subtilis JA show strong pathogen inhibition ability, which has potential market application; yet, the composition of these antibiotics has not been elucidated. The aim of this paper is to isolate and identify these antibiotics. Methods and Results: The antagonistic activity of JA was tested in vitro ; it exhibited strong inhibition against some important phytopathogens and postharvest pathogens. Crude antibiotic production was extracted with methanol from the precipitate by adding 6 mol l?1 HCl to the bacillus-free culture broth. The crude extract was run on Diamonsil C18 column (5 μ m, 250 × 4·6 mm) in HPLC system to separate the antibiotics. Major antibiotics were classified into three lipopeptide families according to electrospray ionization–mass spectrometry analysis. Subsequently, the classification of antibiotics was confirmed with typical collision-induced dissociation fragments. Conclusions: Three kinds of antibiotics were isolated from B. subtilis JA and were identified to the lipopeptide families, surfactin, iturin and fengycin. These compounds could function as biocontrol agents against a large spectrum of pathogens. Significance and Impact of the Study: This study provided a reliable and rapid method for isolation and structural characterization of lipopeptide antibiotics from B. subtilis . |
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Keywords: | antibiotic Bacillus subtilis characterization electrospray ionization mass spectrometry lipopeptide |
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