| MyD88在鼠衣原体生殖道感染过程中的作用 |
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| 引用本文： | 陈丽丽,吴移谋,周洲,雷磊,蔡恒玲,钟光明. MyD88在鼠衣原体生殖道感染过程中的作用[J]. 微生物学通报, 2010, 37(6): 0937-0942 |
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| 作者姓名： | 陈丽丽 吴移谋 周洲 雷磊 蔡恒玲 钟光明 |
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| 作者单位： | 1. 南华大学病原生物学研究所,湖南衡阳,421001
2. Department of Microbiology and Immunology,University of Texas Health Science Center at San Antonio 7703 Floyd Curl Drive,San Antonio,Texas 78229,USA |
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| 基金项目： | 湖南省科技厅基金项目(No. 2009FJ3207); US National Institute of Health 基金项目(No. 1R01 AI47997-01) |
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| 摘 要： | 用1×104IFUs的MoPn经生殖道感染WT、MyD88 KO小鼠,每组一半小鼠于感染后54d,再次感染相同剂量的MoPn。每隔3-4d取生殖道分泌物,测定其中衣原体包涵体的数量。初次感染后80d,处死小鼠,眼眶取血,分离血清,用间接免疫荧光法测其中抗体类型及效价;同时分离生殖道,肉眼观察其输卵管、子宫角水肿程度,并做病理切片观察其炎症反应;分离小鼠脾细胞,体外用衣原体EB刺激,测定产生的IL-4、IL-5、IL-17和IFN-γ等细胞因子水平。MyD88 KO小鼠阴道带菌时间与WT组相当,但上生殖道病理反应,尤其是输卵管水肿程度明显比WT组严重。脾细胞细胞因子水平显示,MyD88 KO鼠IFN-γ和IL-17的产生量明显比WT组低,而IL-4和IL-5水平明显高于WT组。血清中各亚类抗体效价无明显区别,但MyD88 KO鼠血清IgG2a/IgG1比值1,且明显低于WT组。研究结果说明MyD88与抗衣原体免疫无关,但与衣原体引起的炎症损伤密切相关。
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| 关 键 词： | 鼠衣原体(MoPn) MyD88 泌尿生殖道感染 病理 |
The Roles of MyD88 in the Development of Protective Immunity and Pathology During Chlamydial Urogenital Infection in Mice |
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| CHEN Li-Li,WU Yi-Mou,ZHOU Zhou,LEI Lei,CAI Heng-Ling and ZHONG Guang-Ming. The Roles of MyD88 in the Development of Protective Immunity and Pathology During Chlamydial Urogenital Infection in Mice[J]. Microbiology China, 2010, 37(6): 0937-0942 |
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| Authors: | CHEN Li-Li WU Yi-Mou ZHOU Zhou LEI Lei CAI Heng-Ling ZHONG Guang-Ming |
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| Affiliation: | 1. Institute of Pathogenic Biology, Medical College, University of South China, Hengyang, Hunan 421001, China;1. Institute of Pathogenic Biology, Medical College, University of South China, Hengyang, Hunan 421001, China;1. Institute of Pathogenic Biology, Medical College, University of South China, Hengyang, Hunan 421001, China;2. Department of Microbiology and Immunology, University of Texas Health Science Center at San Antonio, 7703 Floyd Curl Drive, San Antonio, Texas 78229, USA;1. Institute of Pathogenic Biology, Medical College, University of South China, Hengyang, Hunan 421001, China;2. Department of Microbiology and Immunology, University of Texas Health Science Center at San Antonio, 7703 Floyd Curl Drive, San Antonio, Texas 78229, USA |
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| Abstract: | |
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| Keywords: | Chlamydia muridarum MyD88 Urogenital tract infection Pathology |
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