Deamination of 5-Hydroxytryptamine by Both Forms of Monoamine Oxidase in the Rat Brain |
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Authors: | Christopher J Fowler Keith F Tipton |
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Institution: | Biochemistry Department, Trinity College, Dublin, Ireland |
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Abstract: | Abstract: K m and V max values of monoamine oxidase (MAO) A and B towards 5-hydroxytryptamine were determined for rat brain homogenates after the in vitro inhibition of one of the two forms by the selective inhibitors clorgyline and l -deprenyl. K m values of 178 and 1170μ m , and V max values of 0.73 and 0.09 nmol·mg protein?1·min?1 towards 5-hydroxytryptamine were found for MAO-A and -B, respectively. The K 1 for 5-hydroxytryptamine as a competitive inhibitor of β-phenethylamine oxidation by MAO-B was found to be 1400 μm. The significance of these findings is discussed. |
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Keywords: | Monoamine oxidase 5-Hydroxytryptamine Rat brain |
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