| 绿豆苯丙氨酸解氨酶的性质及抗肿瘤作用研究 |
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| 引用本文： | 唐煦,周国林.绿豆苯丙氨酸解氨酶的性质及抗肿瘤作用研究[J].微生物学免疫学进展,1996,24(1):35-39. |
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| 作者姓名： | 唐煦 周国林 |
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| 作者单位： | 兰州铁路局中心环境监测站，兰州生物制品研究所疫苗三室 |
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| 摘 要： | 本文参照Ｈａｖｉｒ的方法，从绿豆中分离纯化了苯丙氨酸解氨酶（ＰＡＬ）。纯化的ＰＡＬ经ＳＤＳ聚丙烯酰胺凝胶电泳及等电聚焦电泳鉴定为单一的蛋白质区带，并测得亚基分子量为７６ＫＤ，等电点为５．４５。在ＰＡＬ对Ｌ１２１０小鼠淋巴细胞白血病细胞株的体外抑制实验观察到：ＰＡＬ对该瘤株的抑制作用随作用时间的延长和药物剂量的增加而增强，０．２Ｕ／ｍｌ、１．０Ｕ／ｍｌ、２．０Ｕ／ｍｌ、４．０Ｕ／ｍｌ、６．０Ｕ／ｍｌ、１０．０Ｕ／ｍｌ的ＰＡＬ作用癌细胞７２ｈ，其抑制率分别为２５．８％、４０．０％、５５．３％、７２．６％、７７．９％、８２．９％。
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| 关 键 词： | 苯丙氨酸解氨酶（PAL），绿豆，小鼠淋巴细胞白血病细胞株（L1210） |
Proprety and Anti-Tumor Effect of Phenylalanine Ammonialyase from Sprouting Green Grams |
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| Tang Xu, Zhou Guolin.Proprety and Anti-Tumor Effect of Phenylalanine Ammonialyase from Sprouting Green Grams[J].Progress In Microbiology and Immunology,1996,24(1):35-39. |
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| Authors: | Tang Xu Zhou Guolin |
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| Abstract: | L-Phenylalanine ammonialyase was isolated and purified from sprouting green grams (Phaseolus radiatus L.).Both SDS-PAGE and IEF revealed a single stained protein band which corresponded to a subunit weight of 76 kD and to PI of 5. 45.Antitumor effect showed that the longer six PAI doses acted on murine leukemic lymphoblasts L1210 in vitro,the stronger the inhibitory effect was. The inhibitory rate of six PAL doses (0.2 U/ml, 1.0 U/ml ,2.0 U/ml,4.0 U/ml,6.0 U/ml,10.0 U/ml)at 72h was 25. 8% , 40.0%, 55.3 %, 72.6 %, 77.9%, 82.9% respectively. |
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| Keywords: | L-Phenylalanine ammonialyase (PAL) Green gram Murine leukemic lymphocyte (L1210) |
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