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Photodynamic therapy for American cutaneous leishmaniasis: the efficacy of methylene blue in hamsters experimentally infected with Leishmania (Leishmania) amazonensis |
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| Authors: | Peloi Lílian Somenci Biondo Carlos Eduardo Guerino Kimura Elza Politi Mario José Lonardoni Maria Valdrinez Campana Aristides Sandra Mara Alessi Dorea Regina Coeli Cunha Hioka Noboru Silveira Thaís Gomes Verzignassi |
| Institution: | aDepartamento de Química, Universidade Estadual de Maringá, Maringá, Paraná, Brazil;bDepartamento Farmácia e Farmacologia, Universidade Estadual de Maringá, Maringá, Paraná, Brazil;cInstituto de Química, Universidade de São Paulo, São Paulo, Brazil;dDepartamento de Análises Clínicas e Biomedicina, Universidade Estadual de Maringá, Maringá, Paraná, Brazil;eDepartamento de Ciências Biológicas, Universidade Estadual Paulista, Assis, São Paulo, Brazil |
| Abstract: | The aim of this study was to investigate the effectiveness of Photodynamic Therapy (PDT) using Methylene Blue (MB) as the photosensitizing compound and a Light-Emitting Diode (LED) in American cutaneous leishmaniasis (ACL). Hamsters were experimentally infected with Leishmania (Leishmania) amazonensis. After the development of the lesions in the footpad, the animals were treated with MB three times a week for 3 months. Ten minutes after each application of MB, the lesions were irradiated with LED for 1 h. The lesions were evaluated weekly by the measurement of the hamster footpad thickness. At the end of the treatment the parasitic load was quantified in the regional lymph node of the hamsters. The treatment promoted a decrease in the thickness of infected footpad (P = 0.0001) and reduction in the parasitic load in the regional lymph node (P = 0.0007) of the animals from group treated with MB + LED. PDT using MB + LED in ACL caused by L. amazonensis shows a strong photodynamic effect. This therapy is very promising, once it is an inexpensive system and the own patient can apply it in their wound and in their house without the need of technical assistance. |
| Keywords: | Cutaneous leishmaniasis Photodynamic Therapy Leishmania amazonensis Methylene Blue Light-Emitting-Diode |
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