Rapid extraction of bacterial genomic DNA with guanidium thiocyanate |
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| Authors: | D.G. Pitcher N.A. Saunders R.J. Owen |
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| Affiliation: | National Collection of Type Cultures, Central Public Health Laboratory, London NW9 5HT, UK;*Division of Microbiological Reagents and Quality Control, Central Public Health Laboratory, London NW9 5HT, UK |
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| Abstract: | A method is described for the rapid isolation and purification of bacterial genomic DNA. A total of 215 bacterial strains representing species of Campylobacter, Corynebacterium, Escherichia, Legionella, Neisseria, Staphylococcus and Streptococcus , were lysed with guanidium thiocyanate. DNA was prepared using just three other reagents and one high-speed centrifugation step. The method, which was applicable to both Gram-positive and Gram-negative bacteria, eliminated endogenous nuclease activity and avoided the need for phenol, RNase and protease treatments. The DNA was of high purity, high molecular mass and double-stranded. |
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