Steroid bioconversion in a novel aqueous two-phase system |
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Authors: | R. A. Santos J. C. G. Caldeira H. M. Pinheiro J. M. S. Cabral |
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Affiliation: | (1) Laboratório de Engenharia Bioquímica, Instituto Superior Técnico, Av. Rovisco Pais, 1096 Lisboa Codex, Portugal |
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Abstract: | A novel aqueous two-phase system, based on polyethyleneglycol (PEG) and monosodium glutamate, was tested for the 1-dehydrogenation of hydrocortisone-based substrates. This system led to higher substrate solubilities and biocatalyst/steroid separation levels when compared with alternative systems. The addition of short-chain monohydric alcohols resulted in higher solubilities and more favourable partition coefficients for the tested substrates. Bioconversion activities in PEG/glutamate systems with 2,5% (v/v) methanol were comparable to those measured in monophasic buffer-methanol medium. |
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