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Induced apogamous sporophytes inPteris dispar andP. semipinnata,and the meiotic behavior in their sporocytes
Authors:Suzue M Kawakami  Michio Ito  Shogo Kawakami
Institution:(1) Seirei High School, Seireicho, 489 Seto, Aichi, Japan;(2) Faculty of Science, Shizuoka University, Ooya, 422 Shizuoka, Japan;(3) Faculty of Education, Aichi University of Education, Igaya, 448 Kariya, Aichi, Japan;(4) Present address: 1-6-16 Shiratori, Togo-cho, Aichi-gun, 470-01 Aichi, Japan
Abstract:Spores ofPteris dispar andP. semipinnata were aseptically cultured in flasks for apogamous sporophyte induction. Calli or cell colonies similar to calli were induced in cultures supplemented with hormones. Sporophytic leaves subsequently developed from them in hormone-free medium and the young sporophytes were raised into plants with sporangia. Since the wild-type plants having 116 chromosomes are tetraploid, the sporophytic plants originating from spores would appear to be diploid (dihaploid). In induced sporophytes ofP. semipinnata, non-homologous chromosomes (58 univalents) were found during the meiotic process in sporocytes. InP. dispar, however, the meiotic cells showed many bivalent chromosomes (maximum 29ll). These results suggest thatP. semipinnata is allotetraploid, whereasP. dispar is autotetraploid.
Keywords:Fern  Induced apogamy  Meiosis            Pteris dispar                      Pteris semipinnata
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