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Effects of NO2 and O3 Alone or in Combination on Kidney Bean Plants (Phaseolus vulgaris L.): Products of 13CO2 Assimilation Detected by 13C Nuclear Magnetic Resonance
Authors:ITO  OSAMU; MITSUMORI  FUMIYUKI; TOTSUKA  TSUMUGU
Abstract:The effect of exposure of kidney bean primary leaves to NO2and O3, alone or in combination, on the fate of 13CO2 assimilatedby photosynthesis was examined by 13C-NMR. There were more than70 peaks appearing in the 13C-NMR spectra for substances extractedfrom leaves with 80% ethanol. The 16 relatively well resolvedpeaks corresponded to signals from three sugars, two organicacids and four amino acids. These signals were used to estimatepool sizes and l3C incorporation. Exposures to NO2 and O3 increased the amounts of sucrose andfructose, but not the incorporation of the 13C label during10 min photosynthesis from 13CO2. This suggests the presenceof photo-synthetically inactive pools of sucrose and fructose.Amounts of glycine and serine, and 13C incorporation into them,were increased by the exposure to the pollutants. The incorporationof 13C into alanine was stimulated by exposure to NO2, but notby exposure to O3 alone. The present study shows that with only simple procedures ofsample preparations 13C-NMR provides information on the productsof photosynthesis in leaves stressed by the two air pollutants. Key words: NO2, O3, Phaseolus vulgaris, CO2 assimilation, 13C-NMR
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