Effect of 2,2'-dipyridyl on accumulation of protoporphyrin IX and its derivatives in yeast mitochondria and plasma membranes |
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Authors: | Strakhovskaya M G Ivanova E V Kolesnikova O A Fraikin G Y |
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Institution: | Department of Biophysics, School of Biology, Lomonosov Moscow State University, Moscow, 119899, Russia. |
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Abstract: | The iron chelator 2,2'-dipyridyl (0.2 mM) more than fourfold increased the concentration of protoporphyrin IX and also of its zinc-containing complex in mitochondria of the yeast Saccharomyces cerevisiae. Protoporphyrin IX and a chlorine derivative of protoporphyrin IX which fluoresces at 670-675 nm were found in isolated plasma membranes of the yeast grown in the presence of 0.2 mM 2,2'-dipyridyl. The accumulation of endogenous porphyrins resulted in intensification of lipid photoperoxidation in mitochondria and plasma membranes and in a dramatically increased sensitivity of the cells to visible light (400-600 nm). The relative contribution of photodestruction of subcellular structures to photoinduced cell inactivation is discussed. |
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