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Effect of immobilization on the stability ofClaviceps purpurea protoplasts |
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| Authors: | R Komel D Rozman A Puc H Sočič |
| Institution: | (1) Institute of Biochemistry, Medical Faculty of the E. Kardelj University, Vrazov trg 2, 61 000 Ljubljana, Yugoslavia;(2) Lek, Pharmaceutical and Chemical Works, Verov kova 57, 61 000 Ljubljana, Yugoslavia;(3) Boris Kidri Institute of Chemistry, Hajdrihova 19, 61 000 Ljubljana, Yugoslavia |
| Abstract: | Summary Protoplasts released with high efficiency from vegetative and productive hyphae ofClaviceps purpurea were immobilized in 2% Ca-alginate. The yield of active immobilized protoplasts depended upon the age of the mycelium from which protoplasts were derived and was found to be 25–43% in comparison with native hyphae. During incubation in a modified production medium immobilized protoplasts were stable for at least 10–12 days. No external growth of regenerated hyphae from spherical beads of alginate gel with entrapped protoplasts was observed for 13–15 days of the batchwise incubation. |
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