A rapid TLC autographic method for the detection of glucosidase inhibitors |
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| Authors: | Salazar Mario O Furlan Ricardo L E |
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| Affiliation: | Catedra de Farmacognosia, Facultad de Ciencias Bioquímicas y Farmacéuticas, Universidad Nacional de Rosario, Suipacha 531, S2002LRK Rosario, Argentina. |
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| Abstract: | A new bioautographic assay suitable for the localisation of beta-glucosidase inhibitors present in a complex matrix is described. Enzyme activity was detected using esculin as the substrate to produce esculetin, which reacts with ferric ion to form a brown complex. |
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| Keywords: | bioautography glucosidase plant extracts |
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