棉铃虫幼虫中肠非糖基磷酯酰肌醇锚着氨肽酶N基因的克隆和测序

通过对棉铃虫Helicoverpa armigera (Hübner)幼虫中肠氨肽酶N的克隆和测序，鉴定了1个氨肽酶N基因APN1，其cDNA序列具有3 220个核苷酸，具有3 042 bp的开放阅读框，编码产生1 014个氨基酸的蛋白质。其推定的氨基酸序列具有氨肽酶N所共有的锌结合模体HEXXHX₁₈E和N末端20个氨基酸的疏水性信号序列，但C末端没有糖基磷酯酰肌醇（glycosylphosphatidylinositol,GPI）锚添加信号序列。该氨肽酶N的cDNA序列已提交GenBank，登录号为AY358034。

Cloning and sequencing of midgut aminopeptidase N without glycosylphosphatidylinositol (GPI) anchor from larvae of Helicoverpa armigera (Hubner)

cDNAs of aminopeptidase N (APN) from Helicoverpa armigera (Hübner) were cloned and sequenced. One aminopeptidase N (APN1) was identified. This aminopeptidase N had 3 220 nucleotides with a 3 042 bp open reading frame that encodes 1 014 amino acids. The putative amino acid sequence shares the consensus zinc binding/gluzincin motif HEXXHX₁₈E and a hydrophobic signal sequence with 20 amino acids in N terminal region common to all aminopeptidases， but no glycosylphosphatidylinositol (GPI) anchor signal sequence in C terminal region of APN1 was found. The sequences reported here had been deposited in the GenBank database (Accession number: AY358034).