Specificity of sugar cane trehalase |
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Authors: | Olga Lilia Fleischmacher Marta Amelia Vattuone Fernando Eduardo Prado Antonio Rodolfo Sampietro |
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Institution: | Instituto de Química Biológica, Facultad de Bioquímica, Química y Farmacia, Universidad Nacional de Tucumán, Chacabuco 461. 4000-S.M. de Tucumán, Argentina |
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Abstract: | An extract containing trehalase and invertase was prepared from apical internodes of sugar cane. The extract hydrolysed three glucosides: maltose, trehalose and sucrose. By reprecipitation with ammonium sulphate, maltase and trehalase activities appear to be due to different enzymes. As was also shown by differential inhibition and activation and by studies on the behaviour of both enzymes during growth, invertase and trehalase activities are attributed to different enzymes whose activities do not overlap. Invertase-free preparations confirm these results. Sucrose is a simple competitive inhibitor of sugar cane trehalase, excluding a regulatory role for this sugar. Sucrose was found at inhibitory levels in the first four apical internodes. A close correlation between sugar cane growth and invertase and trehalase levels was found in the apical internodes. Invertase has the greatest activity during growing, and trehalase reaches a maximum at maturity, prior to the flowering process. The high levels of trehalase in the flower suggest that the enzyme is involved in flowering or in related processes linked to seed formation. |
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Keywords: | Gramineae sugar cane trehalase invertase growth |
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