Urease of Spirulina maxima |
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| Authors: | Nelson Carvajal Mireya Fernández Juan Pablo Rodríguez Margarita Donoso |
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| Institution: | Instituto de Nutrición y Teenología de los Atimentos, Universidad de Chile, Casilla 15138, Santiago 11, Chile |
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| Abstract: | Urease (EC 3.5.1.5) was purified from Spirulina maxima by ammonium sulfate precipitation, DEAE-cellulose chromatography and gel filtration on Sephadex G-200. The enzyme had maximum activity at pH 8.7, a Km for urea of 0.12 mM and a MW of ca 232 000. A MW of 38 000 was determined for the subunits. The enzyme was inactivated by p-hydroxymercuribenzoate. |
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| Keywords: | alga urease purification |
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