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Characterization of 20 microsatellite markers in the plowshare tortoise,Astrochelys yniphora
Authors:Angelo R. Mandimbihasina  Shannon E. Engberg  Gary D. Shore  Emilienne Razafimahatratra  Hafany Tiandray  Richard E. Lewis  Rick A. Brenneman  Edward E. Louis Jr.
Affiliation:(1) Durrell Wildlife Conservation Trust, Lot II Y 49 J Ampasanimalo, BP 8511, 101 Antananarivo, Madagascar;(2) Center for Conservation and Research, Omaha’s Henry Doorly Zoo, 3701 South 10th Street, Omaha, NE 68107, USA;(3) Département de Biologie Animale, Faculté des Sciences, Université d’Antananarivo, BP 906, 101 Antananarivo, Madagascar
Abstract:The plowshare tortoise, Astrochelys yniphora, or Angonoka is one of Madagascar’s land tortoises, living in the vicinity of Baly Bay bamboo scrub in northwest Madagascar. Primary threats to this endangered species are due to poaching for pet trade and the loss and fragmentation of its natural habitat. The number of wild Angonoka is estimated to be ~400. Twenty polymorphic nuclear microsatellite loci were isolated from a genomic DNA on individuals from Andrafiafaly in Baly Bay National Park, Madagascar. Results from this study will help for future studies of the social structure and population dynamics of this species as well as identification of confiscated individuals from the illegal pet trade.
Keywords:Plowshare tortoise  Angonoka   Astrochelys yniphora   Testudinidae  Microsatellites  Madagascar
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