The expression of redox proteins of denitrification inThiosphaera pantotropha grown with oxygen,nitrate, and nitrous oxide as electron acceptors |
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| Authors: | James W. B. Moir David J. Richardson Stuart J. Ferguson |
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| Affiliation: | (1) Department of Biochemistry, University of Oxford, South Parks Road, OX1 3QU Oxford, UK;(2) School of Biological Sciences, University of East Anglia, NR4 7TJ Norwich, UK |
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| Abstract: | The redox proteins and enzymes involved in denitrification inThiosphaera pantotropha exhibited a differential expression in response to oxygen. Pseudoazurin was completely repressed during batch or continuous culture under oxic conditions. Cytochromecd 1 nitrite reductase was also heavily repressed after aerobic growth. Nitrite, nitric oxide, and nitrous oxide reductase activities were detected in intact cells under some conditions of aerobic growth, indicating that aerobic denitrification might occur in some circumstances. However, the rates of denitrification were much lower after aerobic growth than after anaerobic growth. Growth with nitrous oxide as sole electron acceptor mimicked aerobic growth in some respects, implying that expression of parts of the denitrification apparatus might be controlled by the redox state of a component of the electron transport chain rather than by oxygen itself. Nevertheless, the regulation of expression of nitrous oxide reductase was linked to the oxygen concentration. |
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| Keywords: | Thiosphaera pantotropha Aerobic denitrification Nitrite reductase Nitric oxide reductase Nitrous oxide reductase |
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