Catalytic properties of a transglucosidase from Aspergillus niger CCRC 31494 |
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Authors: | Tsong-Rong Yan and Ren-De Chiou |
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Affiliation: | (1) Department of Bioengineering, Tatung Institute of Technology, 40, Chung-Sheng North Road, 3rd. Sec., Taipei, Taiwan, 10451, Republic of China;(2) Graduate School of Chemical Engineering, Tatung Institute of Technology, 40, Chung-Sheng North Road, 3rd. Sec., Taipei, Taiwan, 10451, Republic of China |
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Abstract: | Summary A transglucosidase of Aspergillus niger had hydrolysis and transglucosylation activities toward several types of malto- and isomalto-oligosaccharides. The activity was competitively inhibited by glucose and mannose but was not inhibited by galactose and fructose. The Kis of glucose and mannose were 12.9 mM and 75.9 mM, respectively. The enzyme was stable in storage at -20 °C with 60% (v/v) glycerol and 4 °C for at least 40 days. |
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