Modifications of cell surface sialic acids modulate cell adhesion mediated by sialoadhesin and CD22 |
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| Authors: | Sørge Kelm Roland Schauer Jean-Claude Manuguerra Hans-Jürgen Gross Paul R Crocker |
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| Institution: | (1) Biochemisches Institut II, University of Kiel, Olshausenstraße 40, 24098 Kiel, Germany;(2) Unité d'écologie virale, laboratoire de la gripp, Institut Pasteur, 25/28, rue du Docteur Roux, 75724 Paris Cédex 15, France;(3) Institut für Biochemie II, Universität Heidelberg, Im Neuenheimer Feld 328, 69120 Heidelberg, Germany;(4) ICRF, Institute of Molecular Medicine, University of Oxford, John Radcliffe Hospital, OX3 9DU Headington, Oxford, England |
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| Abstract: | An increasing number of mammalian cell adhesion molecules, including sialoadhesion, CD22 and the family of selectins, have been found to bind cell surface glycoconjugates containing sialic acids. Here we describe how the structural diversity of this sugar influences cell adhesion mediated by the related molecules sialoadhesin and CD22 in murine macrophages and B-cells respectively. We show that the 9-O-acetyl group of Neu5,9Ac2 and theN-glycoloyl residue of Neu5Gc interfere with sialoadhesin binding. In contrast, CD22 binds more strongly to Neu5Gc compared to Neu5Ac. Of two synthetic sialic acids tested, only CD22 bound theN-formyl derivative, whereas aN-trifluoroacetyl residue was accepted by sialoadhesin. The potential significance for the regulation of sialic acid dependent cell adhesion phenomena is discussed.Dedicated to Professor Dr Gerhard Uhlenbruck on the occasion of his 65th birthday. |
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| Keywords: | sialic acid cell adhesion O-acetylation N-glycoloylneuraminic acid N-formylneuraminic acid N-trifluoroacetylneuraminic acid |
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