Superoxide dismutase in strains of the genus Flavobacterium: isolation and characterization |
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Authors: | M. Sanchez-Moreno M. Monteoliva-Sanchez F. Ortega A. Ramos-Cormenzana M. Monteoliva |
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Affiliation: | (1) Seccion de Bioquimica, Instituto Lopez-Neyra de Parasitologia, C.S.I.C., E-18001 Granada, Spain;(2) Departamento de Microbiologia, Facultad de Farmacia, Universidad de Granada, Spain;(3) Departamento de Parasitologia, Facultad de Farmacia, Universidad de Granada, Spain |
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Abstract: | Two electrophoretically different forms of superoxide dismutase, one of them containing manganese-protein and the other iron-protein, were detected in eleven different strains of the genus Flavobacterium. The activities of the different strains were similar to those described for other bacteria. The two molecular forms of the enzyme differed clearly with regard to activity, electrophoretic behaviour, sensitivity to cyanide and peroxide, and NaCl requirement. Both molecular forms were isolated from Flavobacterium halmephilum. Molecular mass absorption spectra, metal content, optimum pH, heat-sensitivity and stability were described. |
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Keywords: | Superoxide dismutase Flavobacterium genus Flavobacterium halmephilum |
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