Specificity of peroxidase-labeled antibodies to rabbit, mouse and human immunoglobulins

The comparative study of different types of conjugates (antirabbit, antimouse and antihuman) has shown that gamma globulin fractions actively interact not only with homologous peroxidase-labelled antibodies, but also with heterologous ones. Cross reactions were most pronounced between antimouse conjugates and the preparations of human gamma globulin and, vice versa, between antihuman conjugate and mouse gamma globulin. The study has shown that the main cause of cross reactions is the presence of common antigenic determinants in the preparations of mouse and human gamma globulin.