Sex hormones affect the production of recombinant Factor IX in CHO and HEK-293 cell lines |
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| Authors: | Nazanin Dadehbeigi Seyed Nasser Ostad Mohammad Ali Faramarzi Mohammad Hossein Ghahremani |
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| Institution: | (1) Department of Toxicology and Pharmacology, Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran, Iran;(2) Department of Pharmaceutical Biotechnology, Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran, Iran |
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| Abstract: | Recombinant human Factor IX (rFIX) was cloned in a mammalian expression vector and transfected into CHO and HEK-293. Treatment
with 10−9 M methyl testosterone increased rFIX production by 30–50% in CHO and HEK clones. However, 10−9 M 17β-oestradiol increased production of rFIX by ~50% in CHO-F7 clone and decreased production by 48% and 37% in CHO-F8 and
HEK-F2-6, respectively. Progesterone treatment inhibited rFIX production in both cell lines. Production of rFIX can thus be
increased by sex hormone treatment and therefore used to enhance biotechnological production in mammalian cells. |
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| Keywords: | Mammalian expression Methyl testosterone 17β -oestradiol Recombinant production |
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