Enhanced vanillin production from recombinant E. coli using NTG mutagenesis and adsorbent resin |
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Authors: | Yoon Sang-Hwal Lee Eun-Gyeong Das Amitabha Lee Sook-Hee Li Cui Ryu Hee-Kyoung Choi Myung-Suk Seo Weon-Taek Kim Seon-Won |
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Institution: | Division of Applied Life Science (BK21) and Forest Science, EB-NCRC and PMBBRC, Gyeongsang National University, Jinju 660-701, Korea. |
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Abstract: | Vanillin production was tested with different concentrations of added ferulic acid in E. coli harboring plasmid pTAHEF containing fcs (feruloyl-CoA synthase) and ech (enoyl-CoA hydratase/aldolase) genes cloned from Amycolatopsis sp. strain HR104. The maximum production of vanillin from E. coli DH5alpha harboring pTAHEF was found to be 1.0 g/L at 2.0 g/L of ferulic acid for 48 h of culture. To improve the vanillin production by reducing its toxicity, two approaches were followed: (1) generation of vanillin-resistant mutant of NTG-VR1 through NTG mutagenesis and (2) removal of toxic vanillin from the medium by XAD-2 resin absorption. The vanillin production of NTG-VR1 increased to three times at 5 g/L of ferulic acid when compared with its wild-type strain. When 50% (w/v) of XAD-2 resin was employed in culture with 10 g/L of ferulic acid, the vanillin production of NTG-VR1 was 2.9 g/L, which was 2-fold higher than that obtained with no use of the resin. |
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