The effect of caffeine on post-replication repair and survival in two L5178Y cell lines with different sensitivities to UV irradiation |
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| Authors: | Małgorzata Walicka Ida Körner Walter Malz Janusz Z. Beer |
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| Affiliation: | 1. Department of Radiation Biology and Health Protection, Institute of Nuclear Research, PL-03-195 Warszawa, Poland;2. Department of Radiation Biophysics, Central Institute of Molecular Biology, Academy of Sciences of the GDR, 1115 Berlin-Buch, German Democratic Republic |
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| Abstract: | 2 Strains of murine lymphoma L5178Y cells that varied from the point of view of sensitivity to UV irradiation (mean lethal doses: 3.6 and 8.5 J/m2 for L5178Y-R and L5178Y-S cells, respectively) also differed with respect to sensitization by caffeine. L5178Y-S cells were sensitized to UV irradiation by 0.75 mM caffeine, whereas in the same conditions L5178Y-R cells were not sensitized. Sedimentation analysis of the newly synthesized DNA indicated UV-induced gap formation in L5178Y-S cells only. The subsequent gap filling was inhibited by caffeine. Exposure to UV irradiation induced no gaps in L5178Y-R cells. However, when caffeine was added immediately after irradiation, DNA with reduced molecular weight was found in irradiated cells of both strains after a 2-h chase. On the other hand, caffeine inhibited elongation of undamaged DNA strands in neither of the 2 cell strains. |
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