Autotrophic denitrification by nitrate-dependent Fe(II) oxidation in a continuous up-flow biofilter |
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| Authors: | Jun Zhou Hongyu Wang Kai Yang Bin Ji Dan Chen Huining Zhang Yuchong Sun Jun Tian |
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| Affiliation: | 1.School of Civil Engineering,Wuhan University,Wuhan,China;2.School of Urban Construction,Wuhan University of Science and Technology,Wuhan,China;3.Northeast Electric Power Design Institute,Changchun,China;4.Central and Southern China Municipal Engineering Design and Research Institute,Wuhan,China |
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| Abstract: | A continuous-upflow biofilter packed with sponge iron was constructed for nitrate removal under an anaerobic atmosphere. Microbacterium sp. W5, a nitrate reducing and Fe(II) oxidizing strain, was added to the biofilter as an inoculum. The best results were achieved when NO3 ?-N concentration was 30 mg/L and Fe2+ was 800 mg/L. Nitrite in influent would inhibit nitrate removal and aqueous Fe2+ resulted in encrustation. Fe(II)EDTA would prevent cells from encrustation and the maximum nitrogen removal efficiency was about 90 % with Fe(II)EDTA level of 1100 mg/L. Nitrate reduction followed first-order reaction kinetics. Characteristics of biofilms were analyzed by X-ray fluorescence spectroscopy. |
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