Enhancement in xylose utilization using <Emphasis Type="Italic">Kluyveromyces marxianus</Emphasis> NIRE-K1 through evolutionary adaptation approach |
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Authors: | Nilesh Kumar Sharma Shuvashish Behera Richa Arora Sachin Kumar |
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Institution: | 1.Biochemical Conversion Division,Sardar Swaran Singh National Institute of Bio-Energy,Kapurthala,India;2.I.K Gujral Punjab Technical University,Kapurthala,India |
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Abstract: | The evolutionary adaptation was carried out on the thermotolerant yeast Kluyveromyces marxianus NIRE-K1 at 45 °C up to 60 batches to enhance its xylose utilization capability. The adapted strain showed higher specific growth rate and 3-fold xylose uptake rate and short lag phase as compared to the native strain. During aerobic growth adapted yeast showed 2.81-fold higher xylose utilization than that of native. In anaerobic batch fermentation, adapted yeast utilized about 91 % of xylose in 72 h and produced 2.88 and 18.75 g l?1 of ethanol and xylitol, respectively, which were 5.11 and 5.71-fold higher than that of native. Ethanol yield, xylitol yield and specific sugar consumption rate obtained by the adapted cells were found to be 1.57, 1.65 and 4.84-fold higher than that of native yeast, respectively. Aforesaid results suggested that the evolutionary adaptation will be a very effective strategy in the near future for economic lignocellulosic ethanol production. |
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