A convenient system for highly specific and sensitive detection of miRNA expression |
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| Authors: | Xiangqi Li Minjie Ni Chaobao Zhang Wubin Ma Yonglian Zhang |
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| Affiliation: | 1.Shanghai Key Laboratory for Molecular Andrology, State Key Laboratory of Molecular Biology, Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological Sciences, Shanghai 200031, China;2.Shanghai Institute of Planned Parenthood Research, Shanghai 200032, China;3.School of Biology and Pharmaceutical Engineering, Wuhan Polytechnic University, Wuhan 430023, China |
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| Abstract: | Since the first miRNA was discovered in 1993, miRNAs have become a hotspot for biological research. In order to feed this demand, a robust method is required to detect miRNA gene expression. Development of a detection method is more difficult for miRNAs than for long RNAs, such as mRNA, owing to their small size. Existing methods have limitations; thus, new methods are required. We describe a new system for detecting miRNA expression, which can distinguish miRNA from its precursor and has single-nucleotide resolution. It has single molecule and multiplex detection potential. It may be performed as a polymerase chain reaction (PCR) method, a blotting method, or a macroarray method according to the analyst''s preference. This personalized system provides a convenient tool for the detection of miRNA gene expression. |
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| Keywords: | miRNA, small RNA, liquid hybridization, avidin– biotin detection system, PCR, color development |
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