Responses of diamondback moth to diverse entomopathogenic fungi collected from non-agricultural habitats – Effects of dose,temperature and starvation |
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| Institution: | 1. The United Graduate School of Agricultural Sciences, Tottori University, 4-101 Koyamacho Minami, Tottori, 680-8553, Japan;2. Faculty of Agriculture, Tottori University, 4-101 Koyamacho Minami, Tottori, 680-8553, Japan;3. Department of Biology, Faculty of Mathematics and Natural Sciences, IPB University, Bogor, 16680, Indonesia;1. Centre of Advanced Study, Department of Botany, University of Calcutta, 35, B.C. Road, Kolkata, 700019, India;2. Department of Botany, Vidyanagar College, West Bengal, 743503, India;3. Department of Botany, Sidho-Kanho-Birsha University, Ranchi Road, Purulia, 723104, India;4. Department of Geology, University of Calcutta, 35, B.C. Road, Kolkata, 700019, India;1. Guangdong Provincial Key Laboratory of Microbial Culture Collection and Application, State Key Laboratory of Applied Microbiology Southern China, Institute of Microbiology, Guangdong Academy of Sciences, Guangzhou, Guangdong, 510070, PR China;2. Guangdong Provincial Key Laboratory of Nutraceuticals and Functional Foods, College of Food Science, South China Agricultural University, Guangzhou, Guangdong, 510642, PR China;1. Departamento de Microbiologia, Universidade Federal de Minas Gerais, Brazil;2. Instituto Antártico Argentino, Buenos Aires, Argentina;3. Laboratório de Microbiologia, Universidade Federal do Tocantins, Porto Nacional, Brazil;4. British Antarctic Survey, NERC, High Cross, Madingley Road, Cambridge, CB3 0ET, United Kingdom;5. Department of Zoology, University of Johannesburg, PO Box 524, Auckland Park, 2006, South Africa;6. Departamento de Botânica, Universidade de Brasília, Brasília, Brazil;7. Departamento de Geografia, Universidade Federal de Minas, Gerais, Belo Horizonte, MG, Brazil;1. Research Centre for Ecosystem Resilience, Australian Institute of Botanical Science, Royal Botanic Gardens and Domain Trust, Mrs Macquaries Rd, Sydney, NSW, 2000, Australia;2. PlantClinic, Australian Institute of Botanical Science, Royal Botanic Gardens and Domain Trust, Mrs Macquaries Rd, Sydney, NSW, 2000, Australia;3. Centre for Sustainable Ecosystem Solutions, School of Earth, Atmospheric and Life Sciences, University of Wollongong, Wollongong, NSW, 2522, Australia;1. Laboratorio de Biología Funcional y Biotecnología (BIOLAB)-CICBA-INBIOTEC-CONICET, Facultad de Agronomía, UNCPBA, Av. República de Italia 780, Azul (7300), Buenos Aires, Argentina;2. Grupo Biotecnología y Recursos Genéticos, EEA INTA Marcos Juárez, Ruta 12 s/n, Marcos Juárez (CP2580), Córdoba, Argentina;3. Instituto Tecnológico Chascomús (INTECH), Universidad Nacional de General San Martín (UNSAM)-Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Av. Intendente Marino Km 8.2, CC 164 (7130) Chascomús, Argentina;4. Centro de Investigaciones Integradas sobre Sistemas Agronómicos Sustentables (CIISAS), Facultad de Agronomía, UNCPBA. Av. República de Italia 780, Azul (7300), Buenos Aires, Argentina |
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| Abstract: | We evaluated the virulence of Beauveria bassiana and Metarhizium isolates from soil collected across different vegetation types in Queensland, against chlorantraniliprole-resistant and insecticide-susceptible diamondback moth (DBM) larvae. Host insecticide resistance status had no effect on susceptibility to the pathogens when conidia were topically applied to larvae in the laboratory, and one B. bassiana isolate was significantly more virulent to larvae than the others (seven days after inoculation). The influence of temperature (15, 20, 25 or 30 °C): (i) at the point of host inoculation with conidia and (ii) when the pathogens had already initiated infection and were proliferating in the host haemocoel, was determined experimentally for its influence on virulence, disease progression, and sporulation. Temperature at inoculation had a greater effect on host insect mortality than it did when the fungus was already proliferating in the host haemocoel. The rearing temperature of hosts prior to inoculation had a greater effect on host susceptibility to disease than starvation of the larvae at the time of inoculation. Our results also show that each fungal isolate has its own temperature relations and that these can vary considerably across isolates, and at different points in the pathogen life cycle (germination and cuticular penetration versus growth in the host haemocoel). Temperature also had an idiosyncratic effect, across isolates and across the variables typically used to assess the potential of fungal entomopathogens as biological control agents (time to death, mortality and sporulation rates). This study demonstrates that in addition to pathogenicity and virulence, the temperature relationships of each fungal isolate when infecting insects needs to be taken into account if we are to understand their ecology and use them effectively in pest management. |
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| Keywords: | Inoculation temperature Disease progression Virulence |
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