The Plasmodium falciparum Translationally Controlled Tumor Protein (TCTP) Is Incorporated More Efficiently into B Cells than Its Human Homologue |
| |
| Authors: | Berenice Calderón-Pérez Beatriz Xoconostle-Cázares Rosalía Lira-Carmona Rosaura Hernández-Rivas Jaime Ortega-López Roberto Ruiz-Medrano |
| |
| Institution: | 1. Department of Biotechnology and Bioengineering CINVESTAV-IPN, Mexico City, Mexico.; 2. Unidad de Investigación Médica en Enfermedades Infecciosas y Parasitarias, CMN Siglo XXI IMSS, México City, Mexico.; 3. Department of Molecular Biomedicine, CINVESTAV-IPN, Mexico City, Mexico.; Université Pierre et Marie Curie, France, |
| |
| Abstract: | Plasmodium falciparum secretes a homologue of the translationally controlled tumor protein (TCTP) into serum of infected individuals, although its role in pathogenesis or virulence is unknown. To determine the effect of P. falciparum TCTP on B cells as compared to human TCTP, fluorescently labeled proteins were incubated on primary cultures of mouse splenic B cells and analyzed by flow cytometry and confocal microscopy. Our results indicate that both recombinant proteins are incorporated into B cells, but differ significantly in their rate and percentage of incorporation, being significantly higher for P. falciparum TCTP. Furthermore, P. falciparum TCTP showed a lower B cell proliferative effect than human TCTP, suggesting a mechanism through which the former could interfere in the host''s immune response. |
| |
| Keywords: | |
|
|
