Enzyme-catalysed synthesis of galactosylated 1D- and 1L-chiro-inositol, 1D-pinitol, myo-inositol and selected derivatives using the beta-galactosidase from the thermophile Thermoanaerobacter sp. strain TP6-B1 |
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Authors: | Hart Joanne B Kröger Lars Falshaw Andrew Falshaw Ruth Farkas Erzsébet Thiem Joachim Win Anna L |
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Institution: | Carbohydrate Chemistry Team, Industrial Research Ltd, PO Box 31-310, Lower Hutt, New Zealand. j.hart@irl.cri.nz |
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Abstract: | The products from the enzymatic beta-D-galactopyranosylation of 1D-chiro-inositol, 1D-pinitol, 1D-3-O-allyl-4-O-methyl-chiro-inositol, 1D-3,4-di-O-methyl-chiro-inositol, 1L-chiro-inositol and myo-inositol in combined yields ranging from 46% to 64% have been obtained using the beta-galactosidase isolated from an anaerobic extreme thermophile, Thermoanaerobacter sp. strain TP6-B1 and p-nitrophenyl beta-D-galactopyranoside as the donor. Analysis of the products from these reactions reveals information about the acceptor preferences of the enzyme. |
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Keywords: | Glycosidases Thermostable enzymes Inositol oligosaccharides Glycosylations Thermoanaerobacter sp |
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