ABA consumption in norway spruce (Picea abies) and white spruce (Picea glauca) somatic embryo cultures |
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Authors: | David I Dunstan Stephanie Berry Cheryl A Bock |
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Institution: | (1) Plant Biotechnology Institute, National Research Council Canada, 110 Gymnasium Place, S7N 0W9 Saskatoon, Saskatchewan |
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Abstract: | Summary We investigated abscisic acid (ABA) metabolism among Norway and white spruce somatic embryo cultures which exhibited differences
in maturation response when placed on racemic abscisic acid (±)-ABA]. Differences in metabolic rate among the spruce genotypes
could affect the ABA pool available for the maturation process, and might therefore be responsible for the differences in
maturation response. The production of cotyledonary (stage 3) somatic embryos in cultures (genotypes) of Norway spruce (PA86:26A
and PA88:25B) and of white spruce (WS1F cryoD and WS46) was compared. In each species pair one of the two genotypes failed
to show stage 3 embryo development (respectively, PA88:25B and WS46). The investigation of ABA metabolism of each species
pair showed that no substantial differences in ABA consumption or in the production of metabolites occurred. In each case
ABA was metabolized to phaseic acid and dihydrophaseic acid over the 42-day culture period, metabolites were recoverable from
the agar-solidified medium, and the sum of residual ABA and metabolites were equivalent to the ABA initially supplied. The
results indicate that the process of ABA metabolism occurs essentially independently of somatic embryo maturation.
NRCC no. 37345. |
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Keywords: | ABA somatic embryo maturation Picea abies Picea glauca metabolism photoisomerism |
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