| 35S标记重组植物钙调素的制备方法 |
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| 引用本文： | 崔素娟,郭晓强,毛国红,赵军峰,孙颖,白娟,马力耕,孙大业.35S标记重组植物钙调素的制备方法[J].生物化学与生物物理进展,2002,29(5):801-805. |
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| 作者姓名： | 崔素娟 郭晓强 毛国红 赵军峰 孙颖 白娟 马力耕 孙大业 |
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| 作者单位： | 河北师范大学分子细胞生物学研究室，石家庄 050016;河北师范大学分子细胞生物学研究室，石家庄 050016;河北师范大学分子细胞生物学研究室，石家庄 050016;河北师范大学分子细胞生物学研究室，石家庄 050016;河北师范大学分子细胞生物学研究室，石家庄 050016;河北师范大学分子细胞生物学研究室，石家庄 050016;河北师范大学分子细胞生物学研究室，石家庄 050016;河北师范大学分子细胞生物学研究室，石家庄 050016 |
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| 基金项目： | 国家重点基础研究资助项目（G1999011702）和国家杰出青年基金资助项目（30025024）. |
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| 摘 要： | 利用35S标记的氨基酸混合物喂养工程菌，成功地制备了35S标记的拟南芥钙调素亚型2（35S-ACaM2），对其纯度、放射活度、电泳行为及其灵敏性等进行了检测.结果表明从工程菌中制备的35S-ACaM2纯度高、放射活度高、Ca2+与EGTA存在时的电泳行为与未标记的ACaM2相同，可作为一种高灵敏性的探针用于检测钙调素结合蛋白.
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| 关 键 词： | 35S标记的拟南芥钙调素亚型2(35S-ACaM2)，工程菌，拟南芥 |
| 收稿时间： | 2002/1/15 0:00:00 |
| 修稿时间： | 3/6/2002 12:00:00 AM |
Preparation of Plant Recombinant 35S-Calmodulin in E.coli |
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| CUI Su-Juan,GUO Xiao-Qiang,MAO Guo-Hong,ZHAO Jun-Feng,SUN Ying,BAI Juan,MA Li-Geng and SUN Da-Ye.Preparation of Plant Recombinant 35S-Calmodulin in E.coli[J].Progress In Biochemistry and Biophysics,2002,29(5):801-805. |
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| Authors: | CUI Su-Juan GUO Xiao-Qiang MAO Guo-Hong ZHAO Jun-Feng SUN Ying BAI Juan MA Li-Geng and SUN Da-Ye |
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| Institution: | Laboratory of Molecular and Cell Biology, Hebei Normal University, Shijiazhuang 050016, China;Laboratory of Molecular and Cell Biology, Hebei Normal University, Shijiazhuang 050016, China;Laboratory of Molecular and Cell Biology, Hebei Normal University, Shijiazhuang 050016, China;Laboratory of Molecular and Cell Biology, Hebei Normal University, Shijiazhuang 050016, China;Laboratory of Molecular and Cell Biology, Hebei Normal University, Shijiazhuang 050016, China;Laboratory of Molecular and Cell Biology, Hebei Normal University, Shijiazhuang 050016, China;Laboratory of Molecular and Cell Biology, Hebei Normal University, Shijiazhuang 050016, China;Laboratory of Molecular and Cell Biology, Hebei Normal University, Shijiazhuang 050016, China |
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| Abstract: | 35S-ACaM2 was producted by using 35S-labeled amino acid mixture in E.coli. SDS-PAGE and autoradiograph indicated that high-purified, high-specific radioactivity 35S-ACaM2 was obtained.Electrophoresis character of 35S-ACaM2 is the same as that of unlabeled ACaM2 with Ca2+ or EGTA. Dot-blot and NC overlay experiment showed that 35S-ACaM2 could be used to detect calmodulin-binding proteins as a sensitive probe. |
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| Keywords: | 35S-ACaM2 plant E coli |
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