Active synthesis of glycosaminoglycans by liver parenchymal cells in primary culture |
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Authors: | Yoshifumi Ninomiya Ryu-Ichiro Hata Yutaka Nagai |
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Affiliation: | Department of Tissue Physiology, Medical Research Institute, Tokyo Medical and Dental University, Kandasurugadai, Chiyoda-ku, Tokyo 101 Japan |
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Abstract: | Rat liver parenchymal cells were evaluated after 2 days of primary culture for their ability to synthesize and accumulate heparan sulfate as the major component and low-sulfated chondroitin sulfate, dermatan sulfate, chondroitin sulfate and hyaluronic acid as the minor ones. The newly synthesized glycosaminoglycans secreted into the medium were different from those remaining with and/or on the cell layer. Low-sulfated chondroitin 4-sulfate, a major glycosaminoglycan in blood, was synthesized in the order of 320 μg/liver per day, more than 90% of which was secreted into the medium within 16 h and 40% of the glycan secreted was degraded during that time. On the other hand, heparan sulfate, the major glycosaminoglycan synthesized by the parenchymal cells, was mainly distributed in the cell layer. After 8 days of culture, the synthesis of glycosaminoglycans by the cells increased markedly, especially dermatan sulfate, chondroitin sulfate and hyaluronic acid. |
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Keywords: | Glycosaminoglycan synthesis Sulfation Chondroitin sulfate Heparan sulfate (Liver parenchymal cell) LSC low-sulfated chondroitin sulfate EGTA |
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